Journal: Hypertension (Dallas, Tex. : 1979)

Article Title: Mild Hyperuricemia Attenuates Salt-Sensitive Hypertension and Kidney Damage

doi: 10.1161/HYPERTENSIONAHA.125.25550

Figure Lengend Snippet: Oxidative stress in the kidney. A , Differentially expressed (DE) genes known to affect the synthesis of reactive oxygen species (ROS) and oxidative stress in male rats. B , Western blot for AKR1B10 expression in the kidney and its quantification in male and female rats. C , Representative images of paraffin-embedded 8-OxodG-stained kidney and the quantification of the fluorescence intensity of the staining in male age-matched Sprague-Dawley (SD) rats fed a normal diet (healthy control), Dahl SS rats fed a high-salt (HS) diet, or Dahl SS rats fed a HS/oxonic acid diet. D , Representative images of optimal cutting temperature (OCT)-embedded 8-OxodG-stained kidney of the female Dahl SS rats fed a HS diet, or Dahl SS rats fed a HS/oxonic acid diet. Red-8OxodG, blue- nuclei. Values are shown as mean±SEM. An unpaired t test was used. P value **<0.01, ****<0.0001. Parts of the figure are created using BioRender.com.

Article Snippet: The membrane was first incubated with primary antibody for AKR1B10 (Cat# 18252-1-AP, Proteintech, Rosemont, IL) at 1:5000, then with a secondary antibody (Cat# 711-035-152, Peroxidase AffiniPure Donkey Anti-Rabbit IgG, Jackson ImmunoResearch Laboratories Inc., West Grove, PA) at 1:10000 in 2% BSA in Tris-buffered saline with Tween-20 (TBST).

Techniques: Western Blot, Expressing, Staining, Fluorescence, Control

Journal: Scientific Reports

Article Title: In vitro hyperspectral biomarkers of human chondrosarcoma cells in nanoparticle-mediated radiosensitization using carbon ions

doi: 10.1038/s41598-023-41991-9

Figure Lengend Snippet: Hyperspectral images of CHS cells: incubated with NPs ( a ) treated with NPs and irradiated with carbon ions at 2 Gy ( b ) and 4 Gy ( c ) or with X-rays at 2 Gy ( d ) and 4 Gy ( e ).

Article Snippet: Hyperspectral images (HSI) acquired in enhanced dark field microscopy were obtained using a CytoViva R system (CytoViva Inc., USA).

Techniques: Incubation, Irradiation

Journal: Scientific Reports

Article Title: In vitro hyperspectral biomarkers of human chondrosarcoma cells in nanoparticle-mediated radiosensitization using carbon ions

doi: 10.1038/s41598-023-41991-9

Figure Lengend Snippet: Hyperspectral images of CHS cells: untreated ( a ) irradiated with carbon ions at 2 Gy ( b ) and 4 Gy ( c ) irradiated with X-rays at 2 Gy ( d ) and 4 Gy ( e ).

Article Snippet: Hyperspectral images (HSI) acquired in enhanced dark field microscopy were obtained using a CytoViva R system (CytoViva Inc., USA).

Techniques: Irradiation

Journal: Advances in Experimental Medicine and Biology

Article Title: Hyperspectral Imaging of the Hemodynamic and Metabolic States of the Exposed Cortex: Investigating a Commercial Snapshot Solution

doi: 10.1007/978-3-319-91287-5_3

Figure Lengend Snippet: ( a ) Picture of the setup used for the investigation with the hyperspectral snapshot solution, highlighting the major components; ( b ) RGB microscope picture of the exposed cerebral cortex; ( c ) Spectrally averaged image of the same cortex, acquired during normoxic baseline; ( d ) hyperspectral image of the exposed cortex at 650 nm; ( e ) hyperspectral image of the exposed cortex at 750 nm

Article Snippet: A hyperspectral snapshot cameraHyperspectral imaging (HSI) based on Cubert GmbH technology (the S185 FireflEYE [ ]) was chosen for the study, due to its technical characteristics: primarily, the number of wavelength bands (138) and its spectral resolution (~4 to 8 nm) and sampling (4 nm).

Techniques: Microscopy

Journal: Frontiers in Immunology

Article Title: Influence of metallic particles and TNF on the transcriptional regulation of NLRP3 inflammasome-associated genes in human osteoblasts

doi: 10.3389/fimmu.2024.1397432

Figure Lengend Snippet: Influence of short-term particle exposure on cell morphology of human osteoblasts and influence of particle incorporation on hyperspectral data. (Left) Combined fluorescence darkfield images of osteoblasts exposed to CoCr particles at different time points were taken with the CytoViva ® microscope system. The actin cytoskeleton of cells was stained with phalloidin (green fluorescence), and the nuclei were counterstained with DAPI (blue fluorescence). (Top right) To determine particle uptake, hyperspectral images (HSI) of CoCr particles in cell culture medium and of particles after incubation with cells were taken with the CytoViva ® microscope system, and the mean spectral profiles are shown in a spectral library plot. Bar: 50 µm; 100 µm (CoCr particles).

Article Snippet: Hyperspectral images and data were captured using an optical microscope (Olympus BX 41) equipped with an advanced darkfield illumination system and integrated hyperspectral imaging (HSI) spectrometer (CytoViva Inc., Auburn, AL, USA).

Techniques: Fluorescence, Microscopy, Staining, Cell Culture, Incubation